Inactivation of Saccharomyces pastorianus by MBCOub>2ub> was related to temperature or pressure.
The pHex of S. pastorianus significantly decreased even by MBCOub>2ub> at low temperature.
The pHin of S. pastorianus decreased by increasing temperature or pressure of MBCOub>2ub>.
PI uptake ratio of S. pastorianus increased with temperature or pressure of MBCOub>2ub>.