Identifying promoters for gene expression in Clostridium thermocellum
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- 作者：Daniel G. Olsona ; d ; dan268@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Marybeth Maloneya ; d ; Anthony A. Lanahana ; d ; Shuen Hona ; d ; Loren J. Hauserb ; d ; Lee R. Lynda ; b ; c ; d ; Lee.R.Lynd@dartmouth.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Promoter ; Copy number ; Structural instability ; Rolling circle replication ; lacZ ; adhB
- 刊名：Metabolic Engineering Communications
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：2
- 期：Complete
- 页码：23-29
- 全文大小：2132 K
文摘
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Tested 17 different putative promoter sequences with 2 different reporter genes.
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The eno, cbp, cbp_2, 815, 966, 2638 and 2926 promoters resulted in high enzymatic activity.
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The gapDH, gapDH_2, 1194 and 3011_2 promoters apparently exacerbate problems with plasmid instability.
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The 307, 544, 2463, lac and lacUV5 promoters did not result in reporter gene activity.
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Increasing antibiotic selection pressure did not increase expression of the reporter gene.