Sequence information regarding microvariants at four Y-STR loci is reported. Diversity at the palindromic DYF387S1 STR is shaped by non-allelic gene conversion. An excess of diallelic patterns at RM Y-STRs from cell lines was observed. Low intra- and high inter-population diversity values are observed. Ten additional Y-STRs in Yfiler Plus vs. Yfiler resulted in a 15% DC increase.