Enhancing purification efficiency of affinity functionalized composite agarose micro beads using Fe₃O₄ nanoparticles

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• 作者：S. Amiri ; M.R. Mehrnia ; ^{mmehrnia@ut.ac.ir} ; F. Pourasgharian Roudsari
• 关键词：Agarose beads ; Adsorption capacity ; BSA ; BET-BJH analysis ; Cibacrone blue F3GA ; Elemental analysis ; Fe3O4 nanoparticles ; HETP ; Magnetization ; Spatial hindrance
• 刊名：Journal of Chromatography B
• 出版年：2017
• 出版时间：15 January 2017
• 年：2017
• 卷：1041-1042
• 期：Complete
• 页码：27-36
• 全文大小：2040 K
• 卷排序：1041

文摘

Investigating effects of magnetic nanoparticles on adsorption properties of beads. Specific surface area of magnetic beads is significantly more than nonmagnetic ones. Cibacron Blue F3GA (CB) was used as affinity ligand. CB density on magnetic beads is more than nonmagnetic ones. Protein adsorption and separation properties were investigated. The static and dynamic adsorption capacity was influenced by magnetic nanoparticles.