Characterization of QTL for unique agronomic traits of new-plant-type rice varieties using introgression lines of IR64

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• 作者：Analiza G. Tagle^a ; ¹ ; Daisuke Fujita^a ; ² ; Leodegario A. Ebron^a ; Mary Jeanie Telebanco-Yanoria^a ; Kazuhiro Sasaki^a ; ^b ; ³ ; Tsutomu Ishimaru^a ; ^b ; Yoshimichi Fukuta^b ; Nobuya Kobayashi^a ; ^b ; ⁴ ; ^{nobuya@affrc.go.jp" class="auth_mail" title="E-mail the corresponding author}
• 关键词：NPT ; new plant type ; QTL ; quantitative trait loci (locus) ; GF ; grain fertility ; DTH ; days to heading ; CL ; culm length ; PL ; panicle length ; LL ; leaf length ; FLL ; flag leaf length ; LW ; leaf width ; FLW ; flag leaf width ; FSN ; filled spikelet number per panicle ; TSN ; total spikelet number per panicle ; PN ; panicle number per plant ; IRRI ; International Rice Research Institute ; NIL ; near-isogenic line ; IL ; introgression line ; SSR ; simple sequence repeat ; PCR ; polymerase chain reaction
• 刊名：The Crop Journal
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：4
• 期：1
• 页码：12-20
• 全文大小：626 K

文摘

To enhance the yield potential of an elite indica rice cultivar, an introgression (BC₃-derived) line of IR64, YTH288, was developed using a new-plant-type cultivar, IR66215-44-2-3, as a donor parent. YTH288 has agronomically valuable characteristics such as large panicles, few unproductive tillers, and large leaves inherited from NPT. To identify the genetic basis of these traits, we used 167 F₂ plants derived from a cross between IR64 and YTH288 to conduct QTL analysis for five agronomic traits: days to heading (DTH), culm length (CL), flag leaf length (FLL), flag leaf width (FLW), and filled spikelet number per panicle (FSN). Six putative QTL were detected: four on chromosome 4 (for CL, FLL, FLW, and FSN) and two on chromosome 2 (for DTH and FLL). All QTL with the IR66215-44-2-3 allele, except that for FLL on chromosome 2, had positive effects on each trait. To confirm the effects of these putative QTL, we developed NILs with the IR64 genetic background by marker-assisted selection. We observed significant differences in several agronomic traits between IR64 and NILs that carried these QTL on chromosomes 2 and 4. Additionally, four IR64-NILs carrying chromosomal segments derived from different NPT varieties on the long arm of chromosome 4 exhibited similar pleiotropic effects for unique agronomic traits. These NILs can be used as research materials for studying each trait and as breeding materials for yield improvement of indica rice cultivars.