Role of AINTEGUMENTA-like gene NtANTL in the regulation of tobacco organ growth

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• 作者：Bulat Kuluev^a ; ^b ; ^{kuluev@bk.ru" class="auth_mail" title="E-mail the corresponding author} ; Azamat Avalbaev^a ; ^{avalbaev@yahoo.com" class="auth_mail" title="E-mail the corresponding author} ; Elina Nurgaleeva^b ; Alexey Knyazev^a ; Yuriy Nikonorov^a ; Alexey Chemeris^a
• 关键词：NtANTL ; AINTEGUMENTA-like gene of Nicotiana tabacum ; ARGOS ; auxin-regulated gene involved in organ size ; CYCD3 ; 1 ; cyclin CYCLIND3 ; 1 ; CDKB1-1 ; cyclin dependent kinase B1-1
• 刊名：Journal of Plant Physiology
• 出版年：2015
• 出版时间：15 September 2015
• 年：2015
• 卷：189
• 期：Complete
• 页码：11-23
• 全文大小：3592 K

文摘

The Nicotiana tabacum AINTEGUMENTA-like gene (NtANTL), encoding one of AP2/ERF transcription factors, is a putative ortholog of the AtANT gene from Arabidopsis thaliana. In wild-type tobacco plants, the NtANTL gene was expressed in the actively dividing young flowers, shoot apices, and calluses, while the level of its mRNA increased considerably after treatment with exogenous 6-benzylaminopurine, indoleacetic acid and 24-epibrassinolide. We found a positive correlation among the expression levels of NtANTL, cyclin NtCYCD3;1 and cyclin-dependent kinase NtCDKB1-1 genes, suggesting possible molecular links between AINTEGUMENTA and cell cycle regulators in tobacco plants. However, no correlation was observed between NtANTL, NtCYCD3;1 and NtCDKB1-1 expression levels in response to NaCl and ABA. These observations indicate that the transcription factor NtANTL was not involved in the regulation of the cellular response to salinity nor did it affect the expression of NtCYCD3;1 and NtCDKB1-1 when tobacco plants were exposed to salt stress and ABA. In addition, we generated transgenic tobacco plants with both up-regulated and down-regulated expression of the NtANTL gene. Constitutive expression of the NtANTL gene contributed to an increase in the size of leaves and corolla of transgenic plants. Transgenic plants with reduced expression of the NtANTL gene had smaller leaves, flowers and stems, but showed a compensatory increase in the cell size of leaves and flowers. The results show the significance of the NtANTL gene for the control of organ growth by both cell division and expansion in tobacco plants.