The E3 Ubiquitin Ligase BIG BROTHER Controls Arabidopsis Organ Size in a Dosage-Dependent Manner

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• 作者：Sabine Disch ; Elena Anastasiou ; Vijay K. Sharma ; Thomas Laux ; Jennifer C. Fletcher ; Michael Lenhard
• 关键词：DEVBIO
• 刊名：Current Biology
• 出版年：2006
• 出版时间：7 February 2006
• 年：2006
• 卷：16
• 期：3
• 页码：272-279
• 全文大小：584 K

文摘

Organ growth up to a species-specific size is tightly regulated in plants and animals 1 and 2. Final organ size is remarkably constant within a given species, suggesting that a species-specific size checkpoint terminates organ growth in a coordinated and timely manner. Phytohormones influence plant organ size 3, 4, 5, 6 and 7, but their precise functions in size control are unclear because of their pleiotropic and complex developmental roles 8, 9 and 10. The Arabidopsis transcription factors AINTEGUMENTA and JAGGED promote organ growth by maintaining cellular proliferation potential 11, 12, 13, 14, 15 and 16. Loss of the Antirrhinum transcription factor CINCINNATA causes leaf overgrowth, yet also leads to a highly abnormal leaf shape [17]. Thus, no dedicated factor that limits the final size of plant organs has been isolated. Here, we identify the novel RING-finger protein BIG BROTHER (BB) as a repressor of plant organ growth. Small changes in BB expression levels substantially alter organ size, indicating a central regulatory role for BB in growth control. Recombinant BB protein has E3 ubiquitin-ligase activity that is essential for its in vivo function, suggesting that BB acts by marking cellular proteins for degradation. Our data indicate that plants limit the duration of organ growth and ultimately organ size by actively degrading critical growth stimulators.