The impact of long-term CO₂ enrichment on sun and shade needles of Norway spruce (Picea abies): Photosynthetic performance, needle anatomy and phenolics accumulation

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• 作者：Zuzana Lhotá ; ková ; ^a ; ^{zuza.lhotak@seznam.cz} ; Otmar Urban^b ; ^{urban.o@czechglobe.cz} ; Marianna Dubá ; nková ; ^a ; Milena Cvikrová ; ^c ; ^{cvikrova@ueb.cas.cz} ; Ivana Tomá ; &scaron ; ková ; ^b ; ^{tomaskova.i@czechglobe.cz} ; Lucie Kubí ; nová ; ^d ; ^{kubinova@biomed.cas.cz} ; Karel Zvá ; ra^e ; ^{karel.zvara@mff.cuni.cz} ; Michal V. Marek^b ; ^{marek.mv@czechglobe.cz} ; Jana Albrechtová ; ^a ; ^{albrecht@natur.cuni.cz}
• 关键词：[CO2] ; carbon dioxide concentration ; AC ; ambient CO2 concentration ; ANmax ; light-saturated CO2 assimilation rate ; AQE ; apparent quantum efficiency ; C ; current-year needles ; C  ; +  ; 2 ; 3-year-old needles ; EC ; elevated CO2 concentration ; FACE ; free-ai
• 出版年：2012
• 出版时间：June, 2012
• 年：2012
• 卷：188-189
• 期：Complete
• 页码：60-70
• 全文大小：1209 K

文摘

Norway spruce (Picea abies L. Karst) grown under ambient (365-377 ¦Ìmol (CO₂) mol^?; AC) and elevated (700 ¦Ìmol (CO₂) mol^?; EC) CO₂ concentrations within glass domes with automatically adjustable windows and on an open-air control site were studied after 8 years of treatment. The effect of EC on photosynthesis, mesophyll structure and phenolics accumulation in sun and shade needles was examined. Photosynthetic assimilation and dark respiration rates were measured gasometrically; the structural parameters of mesophyll were determined using confocal microscopy and stereological methods. The contents of total soluble phenolics and lignin were assessed spectrophotometrically, and localizations of different phenolic groups were detected histochemically on needle cross-sections.

EC enhanced the light-saturated CO₂ assimilation rate and reduced dark respiration in the current-year needles. No effects of CO₂ enrichment on mesophyll structural parameters were observed. Similarly, the accumulation and localization of phenolics and lignin remained unaffected by EC treatment. Needles differentiated into sun and shade ecotypes in the same manner and to the same extent irrespective of CO₂ treatment. Based on these results, it is apparent that the EC-induced enhancement of photosynthesis is not related to changes in the examined structural parameters of mesophyll and accumulation of phenolic compounds.