In this work we demonstrate a biochip platform based on Electrochemical Impedance Spectroscopy (EIS), which allows a multiplexed and simultaneous detection of Candida albicans, Streptococcus agalactiae and Chlamydia thracomatis which are three of the most common vaginal infections, in a single device.

The employed transduction method allows a fast, low-cost and label-free detection which is achieved directly from vaginal fluid in around one hour, thus dramatically reducing time for analysis (at least 2-3 days in standard tests).

Practical applications are also demonstrated by analysing 45 real samples from female patients.

This method can result in a great social benefit since the test’s diagnosis can be provided to patients directly at the end of a routine gynaecological check-up.

Moreover, the developed platform is suitable for point-of-care diagnosis of gynaecological infections even in low-resource settings requiring nonskilled staff which is relevant for developing countries, where the spreading of neglected genital infections leads to a number of complications which could involve women and/or their foetus if they are pregnant.