A critical evaluation of how ancient DNA bulk bone metabarcoding complements traditional morphological analysis of fossil assemblages
详细信息 查看全文
- 作者：Alicia C. Grealya ; b ; Matthew C. McDowellc ; Paul Scofieldd ; Dá ; ithí ; C. Murraya ; b ; Diana A. Fuscoc ; James Hailea ; b ; Gavin J. Prideauxc ; Michael Buncea ; b ; michael.bunce@curtin.edu.au" class="auth_mail" title="E-mail the corresponding author
- 关键词：aDNA ; ancient DNA ; Archaeology ; Biodiversity ; Bulk bone ; Experimental error ; Fossil ; Metabarcoding ; Next-generation sequencing ; Palaeontology
- 刊名：Quaternary Science Reviews
- 出版年：2015
- 出版时间：15 November 2015
- 年：2015
- 卷：128
- 期：Complete
- 页码：37-47
- 全文大小：2039 K
文摘
- •
We critically evaluate the utility of the novel bulk bone metabarcoding method.
- •
Several steps in the method significantly contribute to variance in the results.
- •
Families identified molecularly are a subset of those identified morphologically.
- •
The BBM method requires significantly less sampling than traditional methods.
- •
DNA analysis of bulk bone can be a complementary tool for studying the past.