Evaluation of antidiabetic effect of total calystegines extracted from Hyoscyamus albus

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• 作者：Lynda Bourebaba ; ^{lynda06bourebaba@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Souaad Saci ; Damia Touguit ; Lynda Gali ; Schahinez Terkmane ; Naima Oukil ; Fatiha Bedjou
• 关键词：ALAT ; alanine amino transferase ; ANOVA ; analysis of variance ; ASAT ; aspartate amino transferase ; Caly ; calystegines ; CCK ; Cholecystokinin ; DM ; diabetes mellitus ; DNJ ; 1-Deoxynojirimycin ; EC ; enzyme comission ; GL ; amylo-1 ; 6-glucosidase ; Gib ; Glibenclamide ; GP ; glycogen phosphorylase ; HDL ; high density lipoprotein ; IC50 ; inhibition concentration at 50% ; LDL ; low density lipoprotein ; LSD ; least significant difference ; OECD ; Organization for Economic Cooperation and Development ; OGTT ; Oral glucose t
• 刊名：Biomedicine & Pharmacotherapy
• 出版年：2016
• 出版时间：August 2016
• 年：2016
• 卷：82
• 期：Complete
• 页码：337-344
• 全文大小：1780 K

文摘

Hyoscyamus albus L. (Solanaceae) an old medicinal plant is a rich source of tropane and nortropane alkaloids which confers to this plant a number of very interesting and beneficial therapeutic effects.

Purpose

Calystegines that are polyhydroxylated alkaloids and imino-sugars poccess significant glycosidases inhibitory activities and are therefore good candidats for the treatment of diabetes mellitus.

Study design

Calystegines extracted from Hyoscyamys albus seeds were tested for teir acute oral toxicity and investigated for their in-vivo antidiabetic effect on Streptozotocine induced diabetes in mice.

Methodes

Calystegines were extracted from the seeds plant using an Ion exchange column; the remaining extract was then administrated orally to mice at several single doses for acute toxicity assay. A dose of 130 mg/kg streptozotocine was injected to mice to induce diabetes mellitus, and diabetic mice were treated orally during 20 days with 10 mg/kg and 20 mg/kg calystegines and 20 mg/kg glibenclamide as the reference drug.

Results

Acute oral toxicity showed that calystegines are not toxic up to a dose of 2000 mg/kg with absence of any signs of intoxication and damages in Liver and kidney tissues.

The nortropane alkaloids markedly reduced blood glucose levels and lipid parameters of diabetic mice to normal concentrations after 20 days of treatment at 10 mg/kg and 20 mg/ kg (p < 0.05). Histopathological study of diabetic mice pancreas indicated that calystegines of Hyoscyamus albus have minimized streptozotocine damages on β-cells of islets of langerhans, stimulated β-cells regeneration and improved with this insulin secretion.

Conclusion

The findings of this study suggest that calystegines are potent antidiabetic agents with antihyperglicemic and hypolipidemic effects, and a protective fonction on pancreas in streptozotocin induced diabetes in mice.