Promoting scopolamine biosynthesis in transgenic Atropa belladonna plants with pmt and h6h overexpression under field conditions
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- 作者：Ke Xiaa ; 1 ; Xiaoqiang Liua ; 1 ; Qiaozhuo Zhanga ; 1 ; Wei Qianga ; Jianjun Guob ; Xiaozhong Lanc ; Min Chend ; Zhihua Liaoa ; zhliao@swu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; zhihualiao@163.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Atropa belladonna ; Hyoscyamine 6β-hydroxylase ; Putrescine N-methyltransferase ; Metabolic engineering ; Tropane alkaloids
- 刊名：Plant Physiology and Biochemistry
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：106
- 期：Complete
- 页码：46-53
- 全文大小：1719 K
文摘
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T1 progeny of NtPMT-HnH6H overexpressing Atropa belladonna plants showed an impressive chemotype of rich scopolamine.
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Both wild-type and transgenic Atropa belladonna showed the root specific patter of endogenous TA biosynthetic genes.
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Hyoscyamine was converted to scopolamine more efficiently in aerial parts than in underground parts of transgenic plants.