Liquid chromatographic assay of abouthiouzine in plasma and its application to pharmacokinetic studies
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- 作者：Alkharfy ; Khalid M. ; Khan ; Rao Muzaffar A. ; Al-Hadiya ; Badraddin M. ; Abou-Auda ; Hisham S. ; et. al.
- 关键词：Abouthiouzine ; HPLC ; Assay
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2005
- 出版时间：March 25, 2005
- 年：2005
- 卷：817
- 期：2
- 页码：183-186
- 全文大小：124 K
文摘
Abouthiouzine is a newly synthesized antithyroid agent with a proposed less adverse effects profile than other currently used drugs. A simple and rapid reversed phase high performance liquid chromatography assay was developed to determine the concentration of abouthiouzine in human plasma. The procedure involved extraction of the drug and propranolol (internal standard) from the plasma using ethylacetate. The extract was evaporated under nitrogen and the residue was constituted with the mobile phase and injected onto μ-Bondapack phenyl column (10μm, 3.9mm×150mm). The mobile phase consisted of 10mM potassium dihydrogen phosphate buffer, acetonitrile, and methanol in the ratio of 60:25:15 (v/v/v, pH=3.0), which was delivered at a rate of 1.5ml/min. Abouthiouzine and the internal standard were monitored using UV detection at 240nm; the run time was less than 5min. The detection limit of abouthiouzine is 0.5μg/ml. The within- and between-day coefficients of variation were less than 7 % . Our method has been successfully used to measure abouthiouzine plasma concentrations in a rabbit model following an intravenous administration of the drug.