Frequency of HLA-DP-specific antibodies and a possible new cross-reacting group

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• 作者：Carly J. Callender^a ; ^{camato1@jhmi.edu} ; Marcelo Fernandez-Vina^b ; Mary S. Leffell^a ; Andrea A. Zachary^a
• 关键词：HLA-DP ; DP ; CREG
• 刊名：Human Immunology
• 出版年：2012
• 出版时间：February 2012
• 年：2012
• 卷：73
• 期：2
• 页码：175-179
• 全文大小：503 K

文摘

Clinical studies have demonstrated that HLA-DP-specific antibodies can be detrimental to a transplanted kidney. The number of patients affected is proportional to the frequency of DP antibodies. We determined the frequency of HLA-DP-specific antibodies en toto and in the absence of cross-reactive DR antibodies. Of 650 waitlisted renal patients, 271 (42 % ) were reactive with HLA-DP antigens in solid-phase immunoassays. Of these 271 sera, 58 (21 % ) were negative for reactivity with cross-reactive DR antigens, and 16 (5.9 % ) had no class II antibody other than DP. Eliminating sera containing DR cross-reactive antibodies reduced the frequency but not the overall strength of DP antibodies. Although most DP antibodies were not expected to yield a positive cytotoxicity crossmatch, 2 DP-specific antibodies yielded cytotoxic crossmatch tests with titers of >512. The occurrence of HLA-DP-specific antibody differed significantly between previously transplanted (62 % ) and nontransplanted (38 % ) patients, but no difference was observed among patients categorized by race or sex. One serum demonstrated strong cross-reactivity between DP and DRB1*01:03 in the absence of DR1 or DR11 reactivity. Sequence alignments were performed and a possible new cross-reactivity between DRB1*01:03 and DP2, DP9, DP10, DP13, DP16, and DP17 was defined. Two additional sera confirmed this cross-reactivity.