Antibiotic susceptibility of Neochlamydia hartmanellae and Parachlamydia acanthamoebae in amoebae

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• 作者：Manon Vouga^a ; ^b ; Houria Diabi^a ; Areen Boulos^c ; David Baud^a ; Didier Raoult^c ; Gilbert Greub^a ; ^{gilbert.greub@chuv.ch" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Parachlamydiaceae ; Antibiotic ; Pneumonia ; Chlamydia ; Intracellular bacteria
• 刊名：Microbes and Infection
• 出版年：2015
• 出版时间：November-December 2015
• 年：2015
• 卷：17
• 期：11-12
• 页码：761-765
• 全文大小：624 K

文摘

Parachlamydia acanthamoebae and Neochlamydia hartmanellae are Chlamydia-related bacteria naturally infecting free-living amoebae. These strict intracellular bacteria might represent emerging pathogens. Recent studies report an association with lower respiratory tract infections, especially with pneumonia where they have been identified as a potential causative agent in 1–2% of cases. In this study, we defined the antibiotic susceptibility of N. hartmanellae, two strains of P. acanthamoebae and two yet unclassified Parachlamydiaceae strains using a quantitative approach.

We confirmed the results obtained earlier for P. acanthamoebae strain Bn9 in an observational study. Macrolides (MICs < 0.06–0.5 μg/ml), rifampicin (MICs 0.25–2) and doxycycline (2–4 μg/ml) were active against P. acanthamoebae strains and Neochlamydia. All strains were resistant to amoxicillin, ceftriaxone and imipenem (MIC ≥32 μg/ml). Similarly to other Chlamydia-related bacteria, all investigated Parachlamydiaceae were resistant to quinolones (MICs ≥ 16 μg/ml). Therefore, we recommend a treatment with macrolides for Parachlamydia-associated pneumonia.