Detection of an early adult T-cell leukemia-lymphoma clone in lymph nodes with anaplastic lymphoma kinase-negative anaplastic large cell lymphoma involvement

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• 作者：Masahito Tokunaga^a ; ^{ma.tokunaga@jiaikai.jp" class="auth_mail" title="E-mail the corresponding author} ; Noriaki Yoshida^b ; Nobuaki Nakano^a ; Ayumu Kubota^a ; Shogo Takeuchi^a ; Yoshifusa Takatsuka^a ; Masao Seto^b ; Atae Utsunomiya^a
• 关键词：ALK-negative anaplastic large cell lymphoma ; human T-cell leukemia virus type I ; adult T-cell leukemia-lymphoma ; genomic alteration profiles ; T-cell clonality assay
• 刊名：Cancer Genetics
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：209
• 期：4
• 页码：138-142
• 全文大小：587 K

文摘

A 58-year-old man was admitted to our hospital with systemic lymphadenopathy and was diagnosed with anaplastic lymphoma kinase-negative anaplastic large cell lymphoma (ALCL) by lymph node biopsy. Although he was a human T-cell leukemia virus type I (HTLV-1) carrier, Southern blot analysis of the lymph node did not show monoclonal integration of HTLV-1 provirus deoxyribonucleic acid (DNA). He achieved complete remission after chemotherapy and subsequently, autologous peripheral blood stem cell transplantation (auto-PBSCT) was performed. Fifteen months after the auto-PBSCT, abnormal lymphocytes in the peripheral blood gradually increased. Southern blot analysis revealed monoclonal integration of HTLV-1 provirus DNA and monoclonal rearrangement of TRB. He was diagnosed with chronic type adult T-cell leukemia-lymphoma (ATL), which immediately progressed to the acute type. He died of tumor progression despite intensive chemotherapy. We analyzed genomic alterations of the ALCL and ATL cells using array comparative genomic hybridization. We found that the genomic alteration pattern differed between the two diseases. T-cell receptor clonality analysis using polymerase chain reaction (PCR) showed that the T-cell clone of the ATL was present in the lymph nodes with ALCL involvement, but not in peripheral blood. This finding suggests that lymph nodes can serve as a niche for ATL development.