Effect of ionic strength on membrane fouling during ultrafiltration of plasmid DNA

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• 作者：Ying Li ; Kuangzheng Zhu ; Andrew L. Zydney ; ^{zydney@engr.psu.edu}
• 刊名：Separation and Purification Technology
• 出版年：2017
• 出版时间：4 April 2017
• 年：2017
• 卷：176
• 期：Complete
• 页码：287-293
• 全文大小：642 K
• 卷排序：176

文摘

Fouling by plasmid DNA increases dramatically at low ionic strength. Fouling causes large reduction in DNA transmission. Data analyzed using model based on plasmid trapping/partial pore blockage. Model calculations in good agreement with data for plasmid transmission. Fouling rate parameter dependent on effective plasmid size.