Possibilities and limitations of equilibrium sampling using polydimethylsiloxane in fish tissue

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• 作者：Annika Jahnke ; Philipp Mayer ; Dag Broman ; Michael S. McLachlan
• 关键词：In tissue equilibrium sampling ; Silicone thin-film extraction ; Internal exposure ; Solid Phase Microextraction (SPME) ; Persistent organic pollutants (POPs) ; Environmental monitoring
• 刊名：Chemosphere
• 出版年：2009
• 出版时间：October 2009
• 年：2009
• 卷：77
• 期：6
• 页码：764-770
• 全文大小：530 K

文摘

Polydimethylsiloxane (PDMS) has been used for passive equilibrium sampling in numerous abiotic environmental matrices. Recently, this approach was extended to lipid-rich tissue. This work investigated the possibilities and limitations of using PDMS thin-film extraction for in tissue equilibrium sampling in fish species of varying lipid content. Polychlorinated biphenyls (PCBs) were used as model lipophilic organic pollutants. PDMS thin-films were inserted in intact fish tissue for differing time periods (1 h up to 1 week). The thin-films were then solvent-extracted and the extracts were analyzed using gas chromatography coupled to mass spectrometry. Whether equilibrium had been established was investigated either by using PDMS thin-films of multiple thicknesses (140–620 μm) or by assessing kinetics by means of time series. Equilibration was found to be rapid (i.e. in the range of hours) in lipid-rich fish whereas equilibrium was not achieved within one week in tissues with low or medium lipid content (i.e. up to 2 % lipids). Regarding lipid-rich fish, the newly developed method was found to be sufficiently sensitive to determine equilibrium partitioning concentrations of PCBs in lipids of samples from the Baltic Sea, and it is a promising approach for any kind of fatty tissue.