Development of protective agent against Hottentotta saulcyi venom using camelid single-domain antibody
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- 作者：Maryam Darvisha ; Mahdi Behdania ; Mohammad Ali Shokrgozarb ; Kamran Pooshang-Bagheria ; Delavar Shahbazzadeha ; shahbazzadeh@yahoo.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Hottentotta saulcyi ; Nanobody ; Phage display ; Heavy chain antibody (HC-Ab) ; Antivenom
- 刊名：Molecular Immunology
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：68
- 期：2
- 页码：412-420
- 全文大小：1067 K
文摘
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Successful immunization of camel with Iranian Hottentotta saulcyi scorpion venom.
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Venom fractionation using Sephadex G-50 column chromatography to obtain major toxic fraction.
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Preparation of a library from camel heavy chain antibodies using phage display technology.
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Selection a Nanobody (Nb12) capable of recognizing the venom components by phage ELISA.
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Evaluation of protective effect of Nb12 against whole soluble venom injected in mice at 2 LD values in pre-incubated and challenge rescue experiments.