Antivenom production in the alpaca (Vicugna pacos): physiological and antibody responses to monovalent and polyvalent immunisation with Australian elapid venoms

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• 作者：Andrew M. Padula^a ; ^b ; ; Kenneth D. Winkel^b ; ^{andrew.padula@unimelb.edu.au" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Venom ; Antivenom ; Snakebite ; Tiger snake ; Notechis ; Brown snake ; Pseudonaja ; Taipan ; Oxyuranus ; Black snake ; Pseudechis ; Common death adder ; Acanthophis ; Alpaca ; Antibodies ; Camelid ; ELISA
• 刊名：Small Ruminant Research
• 出版年：2016
• 出版时间：August 2016
• 年：2016
• 卷：141
• 期：Complete
• 页码：63-69
• 全文大小：883 K

文摘

Alpaca (Vicugna pacos) are domesticated members of the camelid family that have been shown to produce an IgG subclass that is heavy chain only IgG. This camelid-type IgG has been postulated to have improved neutralising properties and reduced immunogenicity, compared with conventional mammalian IgG. This study was undertaken to describe the physiological and antibody responses of alpaca (Vicugna pacos) used for the production of an experimental camelid antivenom. Various antivenom products were developed by immunisation of alpaca with combinations of five Australian elapid snake venoms: common tiger snake (Notechis scutatus), eastern brown snake (Pseudonaja textilis), Papuan taipan (Oxyuranus scutellatus canni), mulga snake (Pseudechis australis) and common death adder (Acanthophis antarcticus) were emulsified with Freund’s Adjuvant and injected monthly using a low dose multi-site approach. Venom specific immune responses were monitored by ELISA. Physiological effects upon alpaca were monitored by clinical examination combined with serial haematology and biochemical profiles. Serum was harvested once immune responses had peaked and the IgG fraction concentrated by ammonium sulphate precipitate of non-IgG proteins. Total serum IgG and total serum protein concentrations increased and decreased in synchrony with boosting specific antibodies. The difference in serum creatinine kinase concentration measured at 24 h after each of the first three immunisations decreased as the alpaca mounted an antibody response to the venom antigens measured by ELISA. In conclusion, alpaca responded to repeated immunisation with various Australian elapid venoms by mounting an increasing antibody response that persisted following prolonged rest with minimal physiological impact on the animal.