Severe sensitivity loss in an influenza A molecular assay due to antigenic drift variants during the 2014/15 influenza season
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- 作者：Yarah Overmeirea ; Elke Vanlaerea ; Anneleen Hombrouckb ; Hans De Beenhouwerc ; Guus Simonsd ; Antoinette Brinkd ; Anne-Marie Van den Abeelea ; Charlotte Verfailliea ; Jos Van Ackera ; Jos.VanAcker@azstlucas.be" class="auth_mail" title="E-mail the corresponding author
- 关键词：Influenza ; Laboratory ; Laboratory surveillance ; Molecular methods ; Antigenic drift
- 刊名：Diagnostic Microbiology & Infectious Disease
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：85
- 期：1
- 页码：42-46
- 全文大小：514 K
文摘
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During the 2014–2015 influenza season, a decreased sensitivity for the detection of influenza A(H3N2)/3C.2a using a commercially available multiplex assay was encountered.
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Gene sequencing of the influenza A matrix protein showed a point mutation (C163T) leading to a mismatch with the assay probes.
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The known risk of false-negative results in molecular assays due to genomic variation is emphasized, especially in microorganisms subject to great variation such as influenza A.
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Intensive monitoring of incidence, epidemiological data, and clinical context can give a first indication of missed influenza A strains.