Cellulose hydrolysis ability of a Clostridium thermocellum cellulosome containing small-size scaffolding protein CipA
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- 作者：Lan Denga ; Yutaka Moria ; Junjarus Sermsathanaswadia ; Waraporn Apiwatanapiwatb ; Akihiko Kosugia ; b ; akosugi@affrc.go.jp" class="auth_mail" title="E-mail the corresponding author
- 关键词：Cellulosome ; Clostridium thermocellum ; Cohesin ; CelJ ; Crystalline cellulose
- 刊名：Journal of Biotechnology
- 出版年：2015
- 出版时间：20 October 2015
- 年：2015
- 卷：212
- 期：Complete
- 页码：144-152
- 全文大小：1832 K
文摘
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Clostridium thermocellum YM72 that produces small-size CipA (ssCipA) was isolated.
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ssCipA lacked two domains of cohesin 6 and cohesin 7 in CipA.
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Cellulosome using ssCipA decreased cellulose hydrolysis ability.
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CelJ was identified as the exclusively omitted subunit in cellulosome with ssCipA.
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Adequate cooperation of CelJ to cellulosome is important for cellulose hydrolysis.