Efficient saccharification for non-treated cassava pulp by supplementation of Clostridium thermocellum cellulosome and Thermoanaerobacter brockii ¦Â-glucosidase

详细信息    查看全文

• 作者：Pilanee Vaithanomsat^a ; ¹ ; ^{p_vaithanomsat@yahoo.com} ; ^{aappln@ku.ac.th} ; Akihiko Kosugi^b ; ¹ ; ^{akosugi@affrc.go.jp} ; Waraporn Apiwatanapiwat^a ; ^{aapwpa@ku.ac.th} ; Warunee Thanapase^a ; ^{aapwnt@ku.ac.th} ; Rattiya Waeonukul^c ; ^{ping_p2003@hotmail.com} ; Chakrit Tachaapaikoon^c ; ^{chakrit_t@yahoo.com} ; Patthra Pason^c ; ^{pason_pat@yahoo.com} ; Yutaka Mori^b ; ^{ymori@affrc.go.jp}
• 关键词：Saccharification ; Non-treated cassava pulp ; Cellulosome ; Thermophilic ¦Â-glucosidase ; Amylolytic enzymes
• 刊名：Bioresource Technology
• 出版年：2013
• 出版时间：March, 2013
• 年：2013
• 卷：132
• 期：Complete
• 页码：383-386
• 全文大小：264 K

文摘

Cassava pulp containing 60 % starch and 20 % cellulose is a promising renewable source for bioethanol. The starch granule was observed to tightly bind cellulose fiber. To achieve an efficient degradation for cassava pulp, saccharification tests without pre-gelatinization treatment were carried out using combination of commercial ¦Á-amylase with cellulosome from Clostridium thermocellum S14 and ¦Â-glucosidase (rCglT) from Thermoanaerobacter brockii. The saccharification rate for cassava pulp was shown 59 % of dry matter. To obtain maximum saccharification rate, glucoamylase (GA) from C. thermocellum S14 was supplemented to the combination. The result showed gradual increase in the saccharification rate to 74 % (dry matter). Supplementation of GA to the combination of commercial ¦Á-amylase, cellulosome and rCglT is powerful method for efficient saccharification of cassava pulp without pretreatment.