The impact of aminopyrene trisulfonate (APTS) label in acceptor glycan substrates for profiling plant pectin β-galactosyltransferase activities
详细信息 查看全文
- 作者：Stephan Goetz ; Martin Rejzek ; Sergey A. Nepogodiev ; Robert A. Field ; rob.field@jic.ac.uk" class="auth_mail" title="E-mail the corresponding author
- 关键词：Galactosyltransferase ; Pectin ; Acceptor substrate ; Fluorophore ; Capillary electrophoresis
- 刊名：Carbohydrate Research
- 出版年：2016
- 出版时间：4 October 2016
- 年：2016
- 卷：433
- 期：Complete
- 页码：97-105
- 全文大小：1428 K
文摘
- •
APTS-labelled disaccharides serve as acceptor substrates for galactosyltransferases.
- •
Configuration of the alditol linker and site of glycosylation influence GalT turnover.
- •
APTS shows a reaction-promoting effect not evident for similar fluorophores.
- •
β-(1 → 4)-Gal3-APTS acceptor supports enzymatic extension to > 60 galactose residues
- •
Demonstrates the potential of glycan-APTS probes with CE-LIF for CAZyme profiling.