Biosynthesis of uniformly labeled ¹³C- and ¹⁴C-arachidonic acid in Mortierella alpina

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• 作者：Jin V. Lee^a ; Ran Furman^a ; Paul H. Axelsen^a ; ^b ; ^{axe@pharm.med.upenn.edu}
• 关键词：Mortierella alpina ; Arachidonic acid ; Carbon isotope labeling ; Micro-scale culture
• 刊名：Bioresource Technology
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：227
• 期：Complete
• 页码：142-146
• 全文大小：566 K
• 卷排序：227

文摘

Procedures for the laboratory-scale preparation of U-13C-ARA and U-14C-ARA are described. The yield of U-13C-ARA was >100 nmol with an isotopic purity of 95%. The yield of U-14C-ARA was >2 μCi with an estimated specific activity of >1200 Ci/mol. Conversion efficiencies from glucose to ARA were 4–5%. Smaller quantities of other isotope-labeled fatty acids were also obtained.