A study of the small-molecule system used to investigate the effect of arginine on antibody elution in hydrophobic charge-induction chromatography
详细信息 查看全文
- 作者：Atsushi Hiranoa ; 1 ; Takuya Maruyamab ; 1 ; Kentaro Shirakib ; Tsutomu Arakawac ; Tomoshi Kamedad ; kameda-tomoshi@aist.go.jp" class="auth_mail" title="E-mail the corresponding author
- 关键词：Antibody ; Arginine ; Binding free energy ; Molecular dynamics simulation ; Multimodal chromatography
- 刊名：Protein Expression and Purification
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：129
- 期：Complete
- 页码：44-52
- 全文大小：1742 K
文摘
- •
4-mercaptoethylpyridine (4-MEP) adsorbes aromatics such as alkyl gallates.
- •
Arginine is effective in desorption of butyl gallate (BG) from 4-MEP resin.
- •
Molecular dynamics simulation provided the mechanism of the effect of arginine.
- •
Arginine intervenes in the interaction between the BG and 4-MEP molecules.