Molecular cloning and characterization of a flavanone 3-Hydroxylase gene from Artemisia annua L.
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- 作者：Shuo Xionga ; 1 ; Na Tiana ; b ; 1 ; Jinhua Longb ; Yuhong Chena ; Yu Qina ; Jinyu Fenga ; Wenjun Xiaoa ; b ; xiaowenjun88@sina.com" class="auth_mail" title="E-mail the corresponding author ; Shuoqian Liua ; b ; c ; shqianliu@sina.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Flavanone 3-hydroxylase ; Flavonoids ; Naringenin ; Dihydrokaempferol ; Artemisia annua L.
- 刊名：Plant Physiology and Biochemistry
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：105
- 期：Complete
- 页码：29-36
- 全文大小：1147 K
文摘
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A flavanone 3-hydroxylase gene AaF3H was cloned from Artemisia annua for the first time.
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Full-length cDNA of AaF3H was obtained and analyzed.
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AaF3H protein was produced and purified.
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The catalytic property of AaF3H was characterized.
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The relationship between AaF3H and flavonoids level in planta was investigated.