LAB microbiota isolated from an Azorean artisanal cheese was investigated. Enterococcus was the dominant genera identified by gene sequence analyses whereas API system mostly identified Lactobacillus. None of isolates showed high-level resistance to clinically important antibiotics. All Enterococcus isolates were sensitive to vancomycin but presented at least one virulence gene. Two Lactobacillus strains were selected for adjuvant cultures in cheese application.