Oligosaccharide mapping of heparinase I-treated heparins by hydrophilic interaction liquid chromatography separation and online fluorescence detection and electrospray ionization-mass spectrometry characterization
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- 作者：Fabio Galeotti ; Nicola Volpi ; volpi@unimo.it" class="auth_mail" title="E-mail the corresponding author
- 关键词：Heparin ; HILIC ; Oligosaccharide mapping ; Glycosaminoglycans ; Disaccharides ; AMAC ; Heparan sulfate
- 刊名：Journal of Chromatography A
- 出版年：2016
- 出版时间：6 May 2016
- 年：2016
- 卷：1445
- 期：Complete
- 页码：68-79
- 全文大小：2304 K
文摘
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Oligosaccharide mapping of heparins has been performed by HILIC separation.
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Heparin oligosaccharides have been characterized by online mass spectrometry.
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A polar solvent with a high pH has been adopted to avoid oligomers desulfation.
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Nine main heparin oligosaccharides were separated and characterized.
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The content of 3-O-sulfo groups of the ATIII-binding region was determined.