Structural peculiarity and antithrombin binding region profile of mucosal bovine and porcine heparins
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- 作者：Annamaria Naggia ; naggi@ronzoni.it" class="auth_mail" title="E-mail the corresponding author ; Cristina Gardinia ; Giacomo Pedrinolaa ; Lucio Mauria ; Elena Ursoa ; Anna Alekseevaa ; Benito Casua ; Giuseppe Cassinellia ; Marco Guerrinia ; Marcello Iacominib ; Valentina Baigorriac ; Giangiacomo Torria
- 关键词：Bovine heparins ; Nuclear magnetic resonance ; Liquid chromatography ; Mass spectrometry ; ATIII affinity chromatography
- 刊名：Journal of Pharmaceutical and Biomedical Analysis
- 出版年：2016
- 出版时间：25 January 2016
- 年：2016
- 卷：118
- 期：Complete
- 页码：52-63
- 全文大小：2101 K
文摘
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NMR and LC/MS differentiation of bovine and porcine mucosal heparin is provided.
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LC/MS profiles of high ATIII affinity fractions are characteristic fingerprints.
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2-O-Sulfated GlcA was found to be a new NMR marker of bovine mucosal heparin.
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BMH and PMH significantly differ in their content of structural variants of the ATBR.
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ΔU2,4,0 and ΔU4,6,0 fragments are highly represented in bovine affinity fractions.