The role of complement activation in the pathogenesis of Fuchs鈥?dystrophy

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• 作者：脕gnes F眉st ; Dorottya Csuka ; L谩szl贸 Imre ; M谩ria Bausz ; Attila Nagymih谩ly ; Gy枚rgy F眉st ; 脕gnes Csorv谩si ; J谩nos N茅meth ; Lilian Varga
• 关键词：Fuchs&rsquo ; dystrophy ; Corneal endothelium ; Complement activation ; Aqueous humor
• 刊名：Molecular Immunology
• 出版年：April, 2014
• 年：2014
• 卷：58
• 期：2
• 页码：177-181
• 全文大小：524 K

文摘

Purpose

Inflammation can be an etiologic factor of Fuchs鈥?dystrophy according to previous studies. Our aim was to analyse the activation of the complement system in the aqueous humor in this pathological condition.

Methods

100 渭l aqueous humor sample was taken during keratoplasty of 11 Fuchs鈥?dystrophic patients and during phacoemulsification surgery of 18 control patients. The samples were mixed with EDTA and stored at 鈭?0 掳C. Concentrations of C1rC1sC1Inh and C3bBbP complexes as markers of the activation of the classical and alternative complement pathways, respectively, were measured with ELISA method. The results of the patient group and the control group were compared with statistical analysis (non-parametric Mann Whitney test).

Results

Both the concentrations of C1rC1sC1Inh [4.3 (3.2-20.2) AU/ml] and of C3bBbP [15.3 (7.8-22.6) AU/ml] were significantly higher in the Fuchs鈥?dystrophic group than in the control group [C1rC1sC1Inh: 0.0 (0.0-5.6) AU/ml, C3bBbP: 1.4 (0.0-7.8) AU/ml]. The median value is shown along with the (25% and 75% percentiles).

Conclusions

Based on our results, the complement system may be activated both through the classical and alternative pathways in the aqueous humor of the patients with Fuchs鈥?dystrophy.