Monoclonal antibodies to SARS-associated coronavirus (SARS-CoV): Identification of neutralizing and antibodies reactive to S, N, M and E viral proteins

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• 作者：Ralph A. Tripp ; Lia M. Haynes ; Deborah Moore ; Barbara Anderson ; Azaibi Tamin ; Brian H. Harcourt ; Les P. Jones ; Mamadi Yilla ; Gregory J. Babcock ; Thomas Greenough et al.
• 关键词：SARS-coronavirus ; Monoclonal antibody ; Immunoassay ; Epitope ; Neutralizing
• 刊名：Journal of Virological Methods
• 出版年：2005
• 出版时间：2005
• 年：2005
• 卷：128
• 期：1-2
• 页码：21-28
• 全文大小：258 K

文摘

Monoclonal antibodies (Mabs) against the Urbani strain of the SARS-associated coronavirus (SARS-CoV) were developed and characterized for reactivity to SARS-CoV and SARS-CoV S, N, M, and E proteins using enzyme-linked immunoabsorbent (ELISA), radioimmunoprecipitation, immunofluorescence, Western Blot and microneutralization assays. Twenty-six mAbs were reactive to SARS-CoV by ELISA, and nine were chosen for detailed characterization. Five mAbs reacted against the S protein, two against the M protein, and one each against the N and E proteins. Two of five S protein mAbs neutralized SARS-CoV infection of Vero E6 cells and reacted to an epitope within amino acids 490–510 in the S protein. While two of the three non-neutralizing antibodies recognized at second epitope within amino acids 270–350. The mAbs characterized should prove useful for developing SARS-CoV diagnostic assays and for studying the biology of infection and pathogenesis of disease.