Induction of neutralising antibodies and cellular immune responses against SARS coronavirus by recombinant measles viruses

详细信息    查看全文

• 作者：Matthias Liniger ; Arm ; o Zuniga ; Azaibi Tamin ; Teldja N. Azzouz-Morin ; Marlyse Knuchel ; Rene R. Marty ; Marian Wieg ; Sara Weibel ; David Kelvin ; Paul A. Rota ; Hussein Y. Naim
• 关键词：Recombinant vaccines ; Measles virus ; Viral vectors ; SARS
• 刊名：Vaccine
• 出版年：2008
• 出版时间：16 April 2008
• 年：2008
• 卷：26
• 期：17
• 页码：2164-2174
• 全文大小：446 K

文摘

Live attenuated recombinant measles viruses (rMV) expressing a codon-optimised spike glycoprotein (S) or nucleocapsid protein (N) of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) were generated (rMV-S and rMV-N). Both recombinant viruses stably expressed the corresponding SARS-CoV proteins, grew to similar end titres as the parental strain and induced high antibody titres against MV and the vectored SARS-CoV antigens (S and N) in transgenic mice susceptible to measles infection. The antibodies induced by rMV-S had a high neutralising effect on SARS-CoV as well as on MV. Moreover, significant N-specific cellular immune responses were measured by IFN-γ ELISPOT assays. The pre-existence of anti-MV antibodies induced by the initial immunisation dose did not inhibit boost of anti-S and anti-N antibodies. Immunisations comprising a mixture of rMV-S and rMV-N induced immune responses similar in magnitude to that of vaccine components administered separately. These data support the suitability of MV as a bivalent candidate vaccine vector against MV and emerging viruses such as SARS-CoV.