Plasma glutathione and oxidized glutathione level, glutathione/oxidized glutathione ratio, and albumin concentration in complicated and uncomplicated falciparum malaria

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• 作者：Loeki Enggar Fitri¹ ; ^{lukief@ub.ac.id" class="auth_mail" title="E-mail the corresponding author} ; Agustin Iskandar¹ ; Teguh Wahju Sardjono¹ ; Ummu Ditya Erliana² ; Widya Rahmawati² ; Didi Candradikusuma³ ; Utama Budi Saputra³ ; Eko Suhartono⁴ ; Bambang Setiawan⁴ ; Erma Sulistyaningsih⁵
• 关键词：Albumin ; Complicated malaria ; Glutathione ; Oxidized glutathione ; Glutathione/oxidized glutathione ratio ; Uncomplicated malaria
• 刊名：Asian Pacific Journal of Tropical Biomedicine
• 出版年：2016
• 出版时间：August 2016
• 年：2016
• 卷：6
• 期：8
• 页码：646-650
• 全文大小：513 K

文摘

To compare the level of glutathione (GSH) and oxidized glutathione (GSSG), the ratio of GSH/GSSG and the concentration of albumin in plasma of patients with complicated and un-complicated falciparum malaria.

Methods

This research was a cross sectional study using comparison analysis with the plasma GSH and GSSG, the ratio of plasma GSH/GSSG and the concentration of plasma albumin as variables. The complicated malaria patients were obtained from Dr. Saiful Anwar Hospital Malang, whereas uncomplicated malaria patients were obtained from the Regency of Pleihari South Kalimantan. Plasma GSH and GSSG levels were determined by the spectrophotometer at the wave length of 412 nm, whereas the concentration of albumin was determined by bromocresol green method in the pH of 4.1.

Results

There were no significant differences between the level of plasma GSH and GSSG in complicated and uncomplicated malaria patients, as well as the ratio of plasma GSH/GSSG in the two groups (P = 0.373; P = 0.538; and P = 0.615, respectively, independent t-test). In contrast, the plasma albumin concentration in complicated malaria patients were significantly higher than uncomplicated malaria patients (P = 0.000, Mann Whitney U test).

Conclusions

It can be concluded that the average of plasma GSH and GSSG level, also plasma GSH/GSSG ratio in complicated malaria are not different from uncomplicated malaria. Although plasma concentration of albumin in both groups is below the normal range, there is an increase in complicated malaria that might be as compensation of oxidative stress.