Detection of buried Barrett’s glands after radiofrequency ablation with volumetric laser endomicroscopy

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• 作者：Anne-Fré ; Swager ; MD¹ ; David F. Boerwinkel ; MD ; PhD¹ ; Daniel M. de Bruin ; PhD² ; Dirk J. Faber ; PhD² ; Ton G. van Leeuwen ; PhD² ; Bas L. Weusten ; MD ; PhD¹ ; Sybren L. Meijer ; MD ; PhD³ ; Jacques J. Bergman ; MD ; PhD¹ ; ; Wouter L. Curvers ; MD ; PhD⁴
• 关键词：BB ; buried Barrett&rsquo ; s gland ; BE ; Barrett&rsquo ; s esophagus ; EAC ; early adenocarcinoma ; ER ; endoscopic resection ; GEJ ; gastroesophageal junction ; HGD ; high-grade dysplasia ; IM ; intestinal metaplasia ; IQR ; interquartile range ; NBI ; narrow-band imaging ; NSE ; neosquamous epithelium ; OCT ; optical coherence tomography ; OFDI ; optical frequency domain imaging ; RFA ; radiofrequency ablation ; SGS ; subsquamous glandular structure ; VLE ; volumetric laser endomicroscopy ; WLE ; white-light endoscopy
• 刊名：Gastrointestinal Endoscopy
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：83
• 期：1
• 页码：80-88
• 全文大小：2490 K

文摘

The prevalence and clinical relevance of buried Barrett’s glands (BB) after radiofrequency ablation (RFA) in Barrett’s esophagus (BE) are debated. Recent optical coherence tomography studies demonstrated a high prevalence of BBs. Direct histological correlation, however, has been lacking. Volumetric laser endomicroscopy (VLE) is a second-generation optical coherence tomography system capable of scanning a large surface of the esophageal wall layers with low-power microscopy resolution. The aim was to evaluate whether post-RFA subsquamous glandular structures (SGSs), detected with VLE, actually correspond to BBs by pursuing direct histological correlation with VLE images.

Methods

In vivo VLE was performed to detect SGSs in patients with endoscopic regression of BE post-RFA. A second in vivo VLE scan was performed to confirm correct delineation of the SGSs. After endoscopic resection, the specimens were imaged ex vivo with VLE. Extensive histological sectioning of SGS areas was performed, and all histology slides were evaluated by an expert BE pathologist.

Results

Seventeen patients underwent successful in vivo VLE (histological diagnosis before endoscopic treatment: early adenocarcinoma in 8 patients and high-grade dysplasia in 9). In 4 of 17 patients, no SGSs were identified during VLE, and a random resection was performed. In the remaining 13 patients (76%), VLE detected SGS areas, which were all confirmed on a second in vivo VLE scan and subsequently resected. Most SGSs identified by VLE corresponded to normal histological structures (eg, dilated glands and blood vessels). However, 1 area containing BBs was found on histology. No specific VLE features to distinguish between BBs and normal SGSs were identified.

Conclusions

VLE is able to detect subsquamous esophageal structures. One area showed BBs beneath endoscopically normal–appearing neosquamous epithelium; however, most post-RFA SGSs identified by VLE correspond to normal histological structures. (Clinical trial registration number: NTR4056.)