Multicenter experiment for quality control of peptide-centric LC-MS/MS analysis 鈥?A longitudinal performance assessment with nLC coupled to orbitrap MS analyzers
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- 作者：Alex Camposa ; b ; alex.proteomics@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Ramó ; n Dí ; aza ; Salvador Martí ; nez-Bartolomé ; c ; Jose Sierrab ; Oscar Gallardod ; Eduard Sabidó ; e ; Maria Ló ; pez-Lucendof ; J. Ignacio Casalf ; Carla Pasquarellog ; Alexander Scherlh ; Cristina Chivae ; Eva Borrase ; Antonia Odenaa ; Fé ; lix Elortzai ; Mikel Azkargortai ; Nieves Ibarrolaj ; Francesc Canalsk ; Juan P. Albarc ; Eliandre Oliveiraa
- 关键词：Quality control ; LC-MS/MS performance ; Reproducibility ; Yeast
- 刊名：Journal of Proteomics
- 出版年：2015
- 出版时间：8 September 2015
- 年：2015
- 卷：127
- 期：part_PB
- 页码：264-274
- 全文大小：2882 K
文摘
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The LC–MS/MS performance of 6 laboratories was evaluated during 6 months.
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QC metrics were evaluated to check performance of shotgun proteomics workflows.
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Protein identification repeatability overtime is overall fair, IQR: 69.2–72.4%.
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Lack of systematic LC–MS quality assessment can drop identifications down to 30%.