Isolation and identification of inulooligosaccharides resulting from inulin hydrolysis

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• 作者：Sé ; bastien N. Ronkart ; Christophe S. Blecker ; Hé ; lè ; ne Fourmanoir ; Christian Fougnies ; Claude Deroanne ; Jean-Claude Van Herck ; Michel Paquot
• 关键词：Inulin ; Hydrolysis ; Globe artichoke ; Endo-inulinase ; Purification ; Elution profile
• 刊名：Analytica Chimica Acta
• 出版年：2007
• 出版时间：26 November 2007
• 年：2007
• 卷：604
• 期：1
• 页码：81-87
• 全文大小：554 K

文摘

In this study, inulooligosaccharides (F_n-type inulin) resulting from the endo-inulinase hydrolysis of globe artichoke inulin were purified and characterized. The aim was to produce F_n oligomer standards with the intention of identifying them in the complex inulin chromatogram. Inulin was extracted from globe artichoke and presented a high average degree of polymerization (DP) of about 80 as determined by high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD). This inulin was hydrolyzed by a commercial endo-inulinase yielding a product with a very high F_n/GF_n molecule ratio, thus limiting the interference of GF_n during the purification process. High-performance size exclusion chromatography was used to individually isolate and collect each retention peak corresponding to a specific oligomer. The purity of these fractions was checked by HPAEC-PAD and showed that relatively pure molecules were produced. Electrospray ionization mass spectrometry allowed the molecular weight determination of these purified oligomers and ascertained their DP as F₂, F₃ and F₄. These F_2–4 standards were used with glucose, fructose, sucrose and GF_2–4 (commercially available) to spike commercial oligofructose products in order to determine the elution profile in the HPAEC-PAD chromatogram.