Inhibition and conformational change of SERCA3b induced by Bcl-2
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- 作者：Asha Hewarathna ; Elena Dremina ; Christian Schö ; neich ; schoneic@ku.edu
- 关键词：SERCA ; sarco/endoplasmic reticulum Ca2 ; +-ATPase ; Bcl-2 ; B-cell lymphoma 2 ; Bcl-2∆21 ; human recombinant Bcl-2 with the 21 C-terminal amino acids deleted ; ER ; endoplasmic reticulum ; GST ; glutathione S-transferase ; DMEM ; Dulbecco's modified Eagle's medium ; ATP2A3 ; partial recombinant protein of SERCA3 with a GST tag at N-terminal ; FITC ; fluorescein isothiocyanate
- 刊名：Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：1865
- 期：1
- 页码：121-131
- 全文大小：824 K
- 卷排序：1865
文摘
Bcl-2Δ21 interacts with and partially inhibits SERCA3b isoform. Bcl-2 interacts with and partially inhibits SERCA3b in cell culture. Both exogenous Bcl-2Δ21 and endogenous Bcl-2 could form a stable immunocomplex with SERCA3b. Overexpression of Bcl-2 reduced fluorescein isothiocyanate (FITC) labeling of SERCA3b.