Scanning electron microscopy study of new bone formation following small and large defects preserved with xenografts supplemented with pamidronate—A pilot study in Fox-Hound dogs at 4 and 8 weeks

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• 作者：Naroa Lozano-Carrascal^a ; Marta Satorres-Nieto^a ; Rafael Delgado-Ruiz^b ; José ; Eduardo Maté ; -Sá ; nchez de Val^c ; Sergio Alexandre Gehrke^d ; ^{sergio.gehrke@hotmail.com} ; Jorge Gargallo-Albiol^a ; José ; Luis Calvo-Guirado^e
• 关键词：Socket ; Bone grafting ; Biomaterials ; Bone substitutes ; Bisphosphonates ; Pamidronate
• 刊名：Annals of Anatomy - Anatomischer Anzeiger
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：209
• 期：Complete
• 页码：61-68
• 全文大小：5056 K
• 卷排序：209

文摘

The aim of the present study was to evaluate the feasibility of SEM and EDX microanalysis on evaluating the effect of porcine xenografts (MP3®) supplemented with pamidronate during socket healing. Mandibular second premolars (P2) and first molars (M1) were extracted from six Beagle dogs. P2 were categorized as small defects (SD) and M1 as large defects (LD). Four random groups were created: SC (small control defects with MP3®), ST (small test defects MP3® + pamidronate), LC (large control defects with MP3®), and LT (large test defects MP3® + pamidronate). At four and eight weeks of healing the samples were evaluated fisically through scanning electron microscopy (SEM), and chemical element mapping was carried out by Energy dispersive X-ray spectroscopy (EDX). After four weeks of healing, SEM and EDX analysis revealed more mineralized bone in ST and LT groups compared with control groups (p < 0.05). After eight weeks, Ca/P ratios were slightly higher for small defects (groups SC and ST); in SEM description, in both control and test groups, trabecular bone density was similar to the adjacent mineralized cortical bone. Within the limitations of this experimental study, SEM description and EDX elemental microanalysis have demonstrated to be useful techniques to assess bone remodelling of small and large defects. Both techniques show increased bone formation in test groups (MP3® modified with pamidronate) after four and eight weeks of healing.