Comparative whole genome analysis of six diagnostic brucellaphages

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• 作者：Jason Farlow^a ; Andrey A. Filippov^b ; Kirill V. Sergueev^b ; Jun Hang^c ; Adam Kotorashvili^d ; Mikeljon P. Nikolich^b ; ^{mikeljon.p.nikolich.civ@mail.mil" class="auth_mail}
• 关键词：° ; C ; degrees Celsius ; 渭m ; micrometer ; 渭l ; microliter ; acc. no. ; accession number ; BIC ; Bayesian Information Criterion ; Bk ; Berkeley phage ; BS ; bootstrap ; C ; cytidine ; CRISPRs ; clustered regularly interspaced short palindromic repeats ; dN - dS statistic ; the ratio of the number of non-synonymous substitutions per non-synonymous site to the number of synonymous substitutions per synonymous site ; DNA ; Deoxyribonucleic acid ; DnaA ; a protein that activates initiation of DNA replication in prokar
• 刊名：Gene
• 出版年：15 May, 2014
• 年：2014
• 卷：541
• 期：2
• 页码：115-122
• 全文大小：819 K

文摘

Whole genome sequencing of six diagnostic brucellaphages, Tbilisi (Tb), Firenze (Fz), Weybridge (Wb), S708, Berkeley (Bk) and R/C, was followed with genomic comparisons including recently described genomes of the Tb phage from Mexico (Tb_M) and Pr phage to elucidate genomic diversity and candidate host range determinants. Comparative whole genome analysis revealed high sequence homogeneity among these brucellaphage genomes and resolved three genetic groups consistent with defined host range phenotypes. Group I was composed of Tb and Fz phages that are predominantly lytic for Brucella abortus and Brucella neotomae; Group II included Bk, R/C, and Pr phages that are lytic mainly for B. abortus, Brucella melitensis and Brucella suis; Group III was composed of Wb and S708 phages that are lytic for B. suis, B. abortus and B. neotomae. We found that the putative phage collar protein is a variable locus with features that may be contributing to the host specificities exhibited by different brucellaphage groups. The presence of several candidate host range determinants is illustrated herein for future dissection of the differential host specificity observed among these phages.