Optimized subunit vaccine protects against experimental leishmaniasis
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- 作者：Sylvie Bertholet ; Yasuyuki Goto ; Lauren Carter ; Ajay Bhatia ; R ; all F. Howard ; Darrick Carter ; Rhea N. Coler ; Thomas S. Vedvick ; Steven G. Reed
- 关键词：Leishmania ; vaccination ; MPL
- 刊名：Vaccine
- 出版年：2009
- 出版时间：23 November 2009
- 年：2009
- 卷：27
- 期：50
- 页码：7036-7045
- 全文大小：629 K
文摘
Development of a protective subunit vaccine against Leishmania spp. depends on antigens and adjuvants that induce appropriate immune responses. We evaluated a second generation polyprotein antigen (Leish-110f) in different adjuvant formulations for immunogenicity and protective efficacy against Leishmania spp. challenges. Vaccine-induced protection was associated with antibody and T cell responses to Leish-110f. CD4 T cells were the source of IFN-γ, TNF, and IL-2 double- and triple-positive populations. This study establishes the immunogenicity and protective efficacy of the improved Leish-110f subunit vaccine antigen adjuvanted with natural (MPL-SE) or synthetic (EM005) Toll-like receptor 4 agonists.