Investigation on prevalence of Escherichia coli strains carrying virulence genes ipaH, estA, eaeA and bfpA isolated from different water sources

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• 作者：Reza Ranjbar^a ; Sorayya Hosseini^b ; ^{hosseini_soraiya@yahoo.com" class="auth_mail" title="E-mail the corresponding author} ; Taghi Zahraei-Salehi^c ; Roholla Kheiri^d ; Faham Khamesipour^e
• 关键词：Escherichia coli ; Pathotype ; PCR ; Virulence genes ; Water
• 刊名：Asian Pacific Journal of Tropical Disease
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：6
• 期：4
• 页码：278-283
• 全文大小：384 K

文摘

To investigate prevalence of Escherichia coli (E. coli) strains carrying virulence genes ipaH, estA, eaeA and bfpA, isolated from different water sources in Alborz Province.

Methods

This study was carried out in 2014. The research included all E. coli strains isolated from different surface water sources in Alborz Province of Iran. E. coli isolates were detected and identified by standard microbiological and biochemical tests. The strains were evaluated for the presence of virulence genes ipaH, estA, eaeA and bfpA by PCR using specific primers. The PCR amplicons were visualized via electrophoresis and stained with ethidium bromide.

Results

One hundred E. coli strains were isolated and included in the study. The PCR results showed that 97% of the strains harbored ipaH gene. Moreover, estA, eaeA and bfpA genes were found in 37%, 31% and 3% of the isolates.

Conclusions

Our finding showed that the prevalence rates of virulence genes ipaH and estA were very high among E. coli strains isolated from different surface water sources in Alborz Province. Considering their plasmid-borne nature, the risk of transmission of these genes between other bacterial species could pose a high threat to public health.