A facile colorimetric aptamer assay for small molecule detection in food based on a magnetic single-stranded DNA binding protein-linked composite probe
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- 作者：Qian LuanaAuthor Vitae ; Yun XiaAuthor Vitae ; Ning Gana ; ganning@nbu.edu.cn" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae ; Yuting CaoaAuthor Vitae ; Tianhua LiaAuthor Vitae ; Yinji ChenbAuthor Vitae
- 关键词：Colorimetric aptamer assay ; Single-stranded DNA binding protein ; Magnetic SSB-linked probe ; Chloramphenicol ; Core-shell SiO2@Au
- 刊名：Sensors & Actuators: B. Chemical
- 出版年：2017
- 出版时间：February 2017
- 年：2017
- 卷：239
- 期：Complete
- 页码：979-987
- 全文大小：2186 K
文摘
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One facile magnetic SSB-linked aptamer colorimetric assay was developed for specific detection.
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This probe was fabricated based on magnetic SSB (Au MNPs-SSB) microparticles conjugating with detection probe (Apt- SiO2@Au-HRP).
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The composite probe has well signal amplification and only requires one-step replacement reaction for color development.
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The assay based on the composite probe for CAP detection limit can reach to 0.02 ng/mL.