Generation and precise control of dynamic biochemical gradients for cellular assays

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• 作者：Yasushi Saka ; ^{y.saka@abdn.ac.uk} ; Murray MacPherson ; Claudiu V. Giuraniuc
• 关键词：Microfluidics ; Gradient ; Diffusion ; Yeast ; Saccharomyces cerevisiae
• 刊名：Physica A: Statistical Mechanics and its Applications
• 出版年：2017
• 出版时间：15 March 2017
• 年：2017
• 卷：470
• 期：Complete
• 页码：132-145
• 全文大小：2339 K
• 卷排序：470

文摘

A microfluidic device for diffusion-based gradient generation is presented. A unique design prevents the cross-flow between the source and sink channels. The device enables highly dynamic gradients in diffusion chambers. The device’s capacity is demonstrated using yeast cells cultured in a gradient.