Dissecting ITC data of metal ions binding to ligands and proteins
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- 作者：Rachel A. Johnsonb ; Olivia M. Manleya ; Anne M. Spuchesb ; spuchesa@ecu.edu" class="auth_mail" title="E-mail the corresponding author ; Nicholas E. Grossoehmea ; grossoehmen@winthrop.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：ITC ; Metal speciation ; Bioinorganic chemistry ; Metal competition ; Proton competition ; Calorimetry
- 刊名：Biochimica et Biophysica Acta (BBA) - General Subjects
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：1860
- 期：5
- 页码：892-901
- 全文大小：746 K
文摘
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ITC is a powerful tool to study metal–ligand interactions.
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Complex speciation occurs when metal ions are in solution. This speciation can be avoided or mathematically accounted for.
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Mathematical deconvolution of ITC data is a straight-forward process if all coupled equilibria are known.
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Solution competition (K and ΔH) can and should be determined experimentally.
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All thermodynamic state functions derived from ITC data should refer to identical reactions to be comparable.