A kinetic model of one-pot rapid biotransformation of cellobiose from sucrose catalyzed by three thermophilic enzymes

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• 作者：Chao Zhong^a ; Ping Wei^a ; ^{weiping@njtech.edu.cn} ; Yi-Heng Percival Zhang^b ; ^{zhang_yh@tib.cas.cn}
• 关键词：Kinetic modeling ; Cellobiose ; Sucrose ; Cascade biocatalysis ; One-pot biotransformation ; Thermophilic enzyme
• 刊名：Chemical Engineering Science
• 出版年：2017
• 出版时间：6 April 2017
• 年：2017
• 卷：161
• 期：Complete
• 页码：159-166
• 全文大小：1240 K
• 卷排序：161

文摘

Cellobiose is a zero-calorie sweetener and dietary fiber. Value-added cellobiose was synthesized from sucrose by an enzyme cocktail. A kinetic model was developed for data accommodation and process optimization. Use of thermophilic enzymes at elevated temperatures increases reaction rate.