Duplex PCR for the diagnosis of Brucella melitensis and its differentiation from the REV-1 vaccine strain

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• 作者：L.P. Alvarez^a ; ^{alvarez.lucia@inta.gob.ar} ; ^{alvarezlucia@conicet.gov.ar} ; R.B. Marcellino^b ; A. Martinez^b ; C.A. Robles^b
• 关键词：Goats ; Brucella melitensis ; REV-1 ; Molecular diagnosis ; PCR
• 刊名：Small Ruminant Research
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：146
• 期：Complete
• 页码：1-4
• 全文大小：420 K
• 卷排序：146

文摘

The duplex PCR allows the differentiation of B. melitensis field strains from REV-1. This PCR can be implemented for direct molecular diagnosis from tissue samples. The duplex PCR shortens the detection time and has an acceptable cost.