Cloning, overexpression and characterization of a xylanase gene from a novel Streptomyces rameus L2001 in Pichia pastoris
详细信息 查看全文
- 作者：Ran Yanga ; b ; Jinchun Lia ; b ; Chao Tenga ; b ; Xiuting Lia ; c ; lixt@btbu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; lixt@th.btbu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; li_xiuting@163.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Streptomyces rameus L2001 ; Gene cloning ; Recombinant xylanase ; High-level expression
- 刊名：Journal of Molecular Catalysis B: Enzymatic
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：131
- 期：Complete
- 页码：85-93
- 全文大小：2684 K
文摘
- •
In this study, we successfully cloned a novel xylanase gene from Streptomyces rameus L2001 by hiTAIL-PCR.
- •
We illustrated an effective use of a high-cell-density culture to generate a low cost xylanase in BSM medium.
- •
Presumed glycosylation of the recombinant xylanase was confirmed.