Mechanism and stereoselectivity of HDAC I inhibition by (R)-9-hydroxystearic acid in colon cancer

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• 作者：Carola Parolin ; Natalia Calonghi ; Enrica Presta ; Carla Boga ; Paolo Caruana ; Marina Naldi ; Vincenza Andrisano ; Lanfranco Masotti ; Giorgio Sartor
• 关键词：9-HSA ; 9-hydroxystearic acid ; R-9 ; (R)-9-hydroxystearic acid ; S-9 ; (S)-9-hydroxystearic acid ; RT-PCR ; Real time PCR ; HDAC ; histone deacetylase ; HAT ; histone acetyltransferase ; PI ; propidium iodide ; CBP ; chromatin bound proteins
• 刊名：Biochimica et Biophysica Acta (BBA)/Molecular and Cell Biology of Lipids
• 出版年：2012
• 出版时间：October, 2012
• 年：2012
• 卷：1821
• 期：10
• 页码：1334-1340
• 全文大小：855 K

文摘

9-Hydroxystearic acid (9-HSA) belongs to the endogenous lipid peroxidation by-products that decrease in tumors, causing as a consequence the loss of one of the control mechanisms on cell division. It acts as a histone deacetylase (HDAC, E.C 3.5.1.98) inhibitor, and the interaction of the two enantiomers of 9-HSA with the catalytic site of the enzyme, investigated by using a molecular modelling approach, has been reported to be different. In this work we tested out this prediction by synthesizing the two enantiomers (R)-9-HSA (R-9) and (S)-9-HSA (S-9) starting from the natural source methyl dimorphecolate obtained from Dimorphotheca sinuata seeds and investigating their biological activity in HT29 cells. Both enantiomers inhibit the enzymatic activity of HDAC1, HDAC2 and HDAC3, R-9 being more active; R-9 and S-9 inhibitory effect induces an increase in histone H4 acetylation. We also demonstrate that the antiproliferative effect brought about by R-9 is more pronounced as well as we observe increase of p21 transcription and protein content, while the expression of cyclin D1 is decreased. Starting from these observations it can be hypothesized that the interaction of R-9 with HDAC1 induce conformational changes in the enzyme causing loss of its interaction with other proteins, like cyclin D1 itself.