Evaluation and Minimization of Uncertainty in ITC Binding Measurements: Heat Error, Concentration Error, Saturation, and Stoichiometry

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• 作者：Samuel A. Kantonen ; Niel M. Henriksen ; Michael K. Gilson ; ^{mgilson@ucsd.edu}
• 关键词：ITC ; isothermal titration calorimetry ; LS ; least squares ; MC ; Monte Carlo
• 刊名：Biochimica et Biophysica Acta (BBA) - General Subjects
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：1861
• 期：2
• 页码：485-498
• 全文大小：1590 K
• 卷排序：1861

文摘

The uncertainty in injection heat for a VP-ITC microcalorimeter is roughly proportional to heat release Over a wide range of experimental conditions, fixing the stoichiometry, N, reduces uncertainty in calorimetry results, relative to letting N float Reducing the number of titration steps tends to degrade the precision of ITC results Binding thermodynamics can often be precisely fit from low C value plots, given that N is held fixed